Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens.
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چکیده
منابع مشابه
Induction of endogenous murine C-type virus in spleen cell cultures treated with mitogens and 5-bromo-2'-deoxyuridine.
In short-term cultures of BALB/c spleen cells, treatment with a combination of 5-bromo-2'-deoxyuridine (BrdU) and either lipopolysaccharide W. Escherichia coli or concanavalin A resulted in release of C-type virus into the medium. Only lipopolysaccharide induced virus release when given alone. This could be potentiated by a combined treatment with BrdU. In contrast, phytohemagglutinin at mitoge...
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uridine (lUdR) there was an increase in alkaline phosphatase activity after about 24 hr, reaching a maximum usually at about 72 hi. At this concentration, during this time, there was a relatively small inhibition of cell replication. The increment in alkaline phosphatase activity was in large measure prevented by concomitant addition of 10 /¿Mthymidine. Further, lUdR did not increase alkaline ...
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Alkaline phosphatase activity in several cultured primary human intracranial tumor cells varied over a relatively wide range, and there was no correlation between specific activity and the type of tumor from which the cultures were derived. The enzyme was thermolabile, and its activity was strongly inhibited by l-bromotetramisole, levamisole, and L-homoarginine but not by L-phenylalanine and L-...
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Choline chloride produced a dose-dependent induction of alkaline phosphatase activity in HeLa cells. At the highest concentration tested, 40 mM, there was a 5- to 7-fold increase in alkaline phosphatase activity, a significantly greater induction than that produced by equiosmolar additions of either NaCl or sucrose. Enzyme activity was higher than control values by 24 hr after the addition of t...
متن کاملAn Alkaline Phosphatase Reporter Gene Assay for Induction of CYP3A4 In Vitro
CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...
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ژورنال
عنوان ژورنال: Journal of Pharmacobio-Dynamics
سال: 1986
ISSN: 0386-846X,1881-1353
DOI: 10.1248/bpb1978.9.593